Validation of a Commercial Loop-Mediated Isothermal Amplification (LAMP)-Based Kit for the Detection of Salmonella spp. According to ISO 16140:2016

The traditional cultural method (PCR and Real-Time PCR) for Salmonella spp. detection identification is laborious time-consuming. A qualitative LAMP detecting was validated in compliance with ISO 16140:2016. results show a relative accuracy, sensitivity, specificity of 100% comparison the reference 6579-1:2017; LOD50 set as 0.4 CFU/g. Additionally, field study carried out comparing kit, commercially available PCR kit (FoodProof Salmonella, Biotecon Diagnostics), method. suitable reliable spp., simplifying reducing extent steps analytical process. total 105 samples raw poultry meat were screened presence according to three methods: (Enbiotech), FoodProof (Biotecon), Using these methods, only one sample (0.95%) tested positive This further investigated using described 6579-3:2014, order characterise strain. Following this biochemical serological typing, isolate characterised Infantis.